Betatache, AminaChateaux, Jean-françoisJaffrezic-renault, Nicole2023-09-072023-09-0720182170-161X2588-2082http://hdl.handle.net/123456789/15294We report on the deposition of molecularly imprinted polymer (MIP) nanofibers (NFs) by electrospinning and on their potentialities in electrochemical biosensors. Electrospun polymer was the copolymer poly(ethylene-co-vinyl alcohol) denoted EVOH and model imprinted molecule was creatinine. The optimum conditions leading to uniform non-beaded fibers were reported. Infrared spectroscopy was used to establish actual molecular sites creation on the fibers. NF mats deposited on gold electrode were characterized by electrochemical impedance spectroscopy (EIS). The EIS sensor based on creatinine MIP-EVOH NFs (25% of EVOH in DMSO) gave a large linear range for creatinine detection between 1fg/L and 2 mg/L with a low detection limit of 0.015 fg/L. Reproducibility of 1% to 9% on the same sensor, and 1% to 15% from one sensor to another with a very good selectivity for creatinine.enelectrospinningnanofiberspoly(ethylene co-vinyl alcohol)moleculary imprinted polymercreatinineelectrochemical sensorArticle