Benchelia, S.Benosmane, D.Mekki, E.Tabet, N.2023-09-172023-09-1720142170-161Xhttp://hdl.handle.net/123456789/15983ZnO nanoparticles with different grain sizes have been obtained by mechanical milling, after different treatment times: 3, 6, 12 and 24 hours. The final products have been characterized by different analytical techniques, such as X Ray Diffraction, Photoluminescence, Fourier transform infrared (FTIR) and UV-Visible spectroscopies. The cytotoxic effect of one selected concentration ZnO nanoparticles have been evaluated for paramecium growth kinetics and the mortality rate have been recorded as a function of time. Findings demonstrated that the evolution of the paramecium cell number is clearly affected by ZnO Nps presence.enMechanical millingParameciumNanoparticlesZnOAlternative methodArticle