Hplc chromatographic analysis antioxidant activity of seeds some varieties of prickly pear (opuntia ficus-indica l.) from the sidi-fredj souk ahras algeria
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Date
2021-12-13
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Publisher
University of Oum El Bouaghi
Abstract
The study was conducted on seeds of three varieties of Opuntia ficus-indica (O. ficus-indica), harvested from different regions of Sidi-Fredj (Souk Ahras, Northeast of Algeria). The analysis consisted in antioxidants extraction, following the solid-liquid extraction with ethanol 40% (v/v), their quantification and determination of their antioxidant activity by two methods (reducing power and DPPH Test). Results shows that total phenolic content (TPC) ranged from 74.07 to 92.83 mg GAE GAE /100g Extract. The yellow variety has the best rate followed, by the orange and green ones. Concerning, flavonoids and tannins, the orange variety contains higher concentrations corresponding to 2.64 mg QE/100g and 6.61mg CE/100g. activities of extracts of three varieties shows similar performance; the EC50 for the reduction of ferric iron 0.05g/mL for all extracts, whereas the EA more important in yellow and green seeds extracts. Regarding the scavenging of DPPH •, green and yellow varieties show the highest capacity equals to 0.26g/mL. Linear correlations between the different studied activities and some antioxidants (flavonoids, tannins) rates were noticed; indicating their participation on the obtained effect.
Analysis of the oil by HPLC revealed the presence of phenolic compounds including gallic acid and a form of vitamin E (a-tocophé rol), capable of expressing biological activities; Anti-oxidants and vitamins.CPG analysis showed that prickly pear oil was a major source of essential fatty acids (C18:2 and C18:3).
The seeds extract present therefore a variety effect with properties that should be taken intoconsideration for possible other exploitation in addition to the oil one.