Study of the biological activity of B- carboline alkaloids compounds

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dc.contributor.authorBoumaraf, Takwa
dc.contributor.authorAyadi, Djihane
dc.contributor.authorBenbott, Amel
dc.date.accessioned2022-11-15T23:05:25Z
dc.date.available2022-11-15T23:05:25Z
dc.date.issued2022
dc.description.abstractPeganum harmala belongs to the family Zygophylaceae, and it is one of the most widely used plants in Algerian folk medicine in the treatment of jaundice and relieving joint pain and rheumatism. The aim of this study is to contribute to the evaluation of some biological activities of the roots extracts of P.harmala alkaloids, which are grown in Sidi El-Raghis region - Oum El Bouaghi country.Using the classical method of extraction of indole alkaloids, the yield of alkaloids in the roots was estimated at 2.34%. The chromatographic study (TLC) showed that the root extract contains two types of alkaloid compounds represented by harmine and harmaline using a solvent system consisting of (methanol / chloroform / ammonia) (79-20-1)(V). The analysis of antioxidant activity using DPPH and FRAP methods showed that the harmine had a significant activity in capturing free radicals (IC50= 46.89 ± 0.31 µg/ml) with DPPH test, while the crude alkaloid extract had a great ability to reduce Fe 3+ to Fe 2 + , (EC50 = 0.12 ± 0.05 mg/ml) with FRAP test . The results of the anti-inflammatory activity showed For the alkaloids compounds, there were significant differences compared to the control compound represented by diclofenac, the best value was recorded with the harmine compound (EC50= 22.71 ± 0.52 µg/ml). Regarding the anticoagulant activity, the results related to the time effect showed a very important difference with respect to both APTT and PT, where the best values were recorded with harmine, with both tests with (37.94 ± 3.07 seconds), (23.57 ± 2.11 seconds), respectively. The antibacterial activity of different alkaloids extracts was estimated against three reference strains: P. aeruginosa, S. aureus and E. coli using the agar method in nutrient medium. These results showed that the crude alkaline extract of the roots was the strongest against strain S aureus with damping area diameter of 20 mm.ar
dc.identifier.urihttp://hdl.handle.net/123456789/14395
dc.language.isoenar
dc.publisherUniversité Larbi Ben M'hidi Oum El Bouaghiar
dc.subjectAntibacterial activityar
dc.subjectPeganum harmalaar
dc.subjectAnti-inflammatory activityar
dc.titleStudy of the biological activity of B- carboline alkaloids compoundsar
dc.typeOtherar
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قسم علوم الطبيعة والحياة