Browsing by Author "Lahouel, Mesbah"

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    Chemical constituents and antimicrobial activity of essential oils of Ammodaucus leucotricus
    (Elsevier, 2013) Gherraf, Noureddine; Zellagui, Amar; Kabouche, Azeddine; Lahouel, Mesbah; Salhi, Ramdane; Rhouati, Salah
    Ammodaucus belongs to the family Apiaceae (Umbelliferae), subfamily Apioideae, trib Caucalideae, and comprises one species in Algeria (Quezel and Santa, 1962). The biological importance of members of this genus promoted us to reinvestigate the volatile constituents of the fruits of Ammodaucus leucotricus Coss. et Dur., and to carry out a biological study as far as the antibacterial activity is concerned. The present work deals with the chemical composition and antibacterial activities of the hydrodistilled oils. After extraction, the oil was analyzed by gas chromatography–mass spectrometry to afford 14 components. The major components were found to be, 1-cyclohexene-1-carboxaldehyde (56.40%), D-limonene (28.82%), and 2-pentanone- 4-hydroxy-4-methyl (5.73%). Moreover, the antimicrobial activities of the volatile oils were evaluated by disk diffusion method against Gram-positive and Gram-negative bacteria. The results showed a strong antibacterial activity against Staphylococcus aureus, Escherichia coli and Klebsiella pneumonia.
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    Propolis Extract Effect Against Methicillin Resistant Staphylococcus Aureus Mrsa
    (Oum-El-Bouaghi University, 2017) Adoui, Mounira; Boukaloua, Ahmed; Lahouel, Mesbah
    The Methicillin-resistant Staphylococcus aureus (MRSA) bacteria have been considered as a main cause of nosocomial infections in hospitals, there is a real therapeutic interest to investigate natural compounds or extracts which are able to limit this resistance. The aims of this study are the MRSA strains detection and the assessment of their resistance profile against diverse antibiotic families. Besides, the study aims to assess the antibacterial activity of the propolis against these multi-resistant strains. To achieve this purpose, 44 S. aureus strains were isolated and identified by standard tests. Both Antibiotic sensitivity and antibacterial activity of propolis were determined using the Mueller-Hinton agar diffusion method. A low rate of MRSA (20.45%) has been revealed compared to those noted by the 13th and 15th evaluation reports of the Algerian network for observing bacterial resistance to antibiotics (respectively 32.67% and 47.33%). The resistance profile analysis of MRSA against antibiotic confirmed the multi-resistant nature of these bacteria to several antibiotic families, especially aminosides and macrolides. The studied propolis showed a large antibacterial effect against all our MRSA multi-resistant strains. This effect is related to its high total polyphenols and flavonoids levels. Therefore, propolis exhibited real potential in an alternative fight against staphylococcal infections.