Browsing by Author "Jaffrezic-renault, Nicole"

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    Conception And Characterization Of Moleculary Imprinted Polymers Nanofibers Of Poly(ethylene-co-vinyl Alcohol) And Their Use As Membrane In Electrochemical Sensor For Creatinine Detection
    (Oum-El-Bouaghi University, 2018) Betatache, Amina; Chateaux, Jean-françois; Jaffrezic-renault, Nicole
    We report on the deposition of molecularly imprinted polymer (MIP) nanofibers (NFs) by electrospinning and on their potentialities in electrochemical biosensors. Electrospun polymer was the copolymer poly(ethylene-co-vinyl alcohol) denoted EVOH and model imprinted molecule was creatinine. The optimum conditions leading to uniform non-beaded fibers were reported. Infrared spectroscopy was used to establish actual molecular sites creation on the fibers. NF mats deposited on gold electrode were characterized by electrochemical impedance spectroscopy (EIS). The EIS sensor based on creatinine MIP-EVOH NFs (25% of EVOH in DMSO) gave a large linear range for creatinine detection between 1fg/L and 2 mg/L with a low detection limit of 0.015 fg/L. Reproducibility of 1% to 9% on the same sensor, and 1% to 15% from one sensor to another with a very good selectivity for creatinine.
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    Optimization Of The Surface Initiated Polymerization Of Molecular Imprinted Methacrylate Polymer On Gold Electrode For The Detection Of Testosterone
    (Oum-El-Bouaghi University, 2018) Betatache, Amina; Lagarde, Florence; Jaffrezic-renault, Nicole
    In this work a sensor based on moleculary imprinted polymer (MIP) was fabricated to detect testosterone which is an endocrine disruptor. The methacrylic MIP is polymerized on the gold electrode surface, through the grafting of the initiator 2,2’-Azobis(2-Amidinopropane) (APA) on gold surface. A mixed SAM was firstly built from 1-hexanethiol and an alkanethiol bearing carboxyl end groups whose chain length determines the distance of (APA) initiator from the surface of the gold electrode. Ferrocene-carboxylic acid was used to quantify free amine group of the (APA) initiator fixed on mixed SAM without bridging and ferrocenyl methyl methacrylate was used to check through cyclic voltammetry (CV) that methacrylate monomers polymerize on the electrode surface. Using these tools, the SAM composition was optimized as 1-hexanethiol (HT): 11-mercaptoundecanoic acid (AMU) with a molar ration of 50/50. The detection of testosterone with the MIP-sensor was done using electrochemical impedance spectroscopy. A linear range of 1pg/L to 1 ng/L was obtained, with a detection limit of 1 pg/L.