Browsing by Author "Benbott, Amel"
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Item Effect Of Alkaloids Extract Of Peganum Harmala Seeds On Histo-function Of Rat’s Testes(Oum-El-Bouaghi University, 2018) Benbott, Amel; Mahdi, Djahida; Zellagui, Ammar; Moumen, Yasmina; Mosbah, CamiliaThe Peganum harmala plant is commonly used as traditional herbal medicine in many countries including Algeria. The aim of this study was to investigate the effect of alkaloids extract of P. harmala seeds on histo-function of rat’s testes. Twenty adult Albinos rats were divided equally into four groups, and treated intraperitoneally for 30 days: Group I served as control, received water. Group II, group III, and Group IV received daily a single dose of 40, 80, and 120 mg/kg body weight of alkaloids P. harmala extract respectively. Harmine and harmaline were the components identified in total alkaloids seeds extract by using high performance liquid chromatography (HPLC) method. Body weight, testes relative weights, and testosterone serum levels decreased significantly in group III (P< 0.05) and group IV (P<0.01), whereas levels of LH hormone decreased significantly only (P<0.01) in group IV. The histological alterations were seen in group III and IV, but they were severe in group IV. They mainly included stop of spermatogenesis, deformation in seminiferous tubules structure, damage to leydig cells, and absence of Sertoli cells. In conclusion, P. harmala alkaloids induced testicular toxicity at higher doses, and disturbed the function of hypothalamic pituitary testis axis, which caused infertility.Item Study of the biological activity of B- carboline alkaloids compounds(Université Larbi Ben M'hidi Oum El Bouaghi, 2022) Boumaraf, Takwa; Ayadi, Djihane; Benbott, AmelPeganum harmala belongs to the family Zygophylaceae, and it is one of the most widely used plants in Algerian folk medicine in the treatment of jaundice and relieving joint pain and rheumatism. The aim of this study is to contribute to the evaluation of some biological activities of the roots extracts of P.harmala alkaloids, which are grown in Sidi El-Raghis region - Oum El Bouaghi country.Using the classical method of extraction of indole alkaloids, the yield of alkaloids in the roots was estimated at 2.34%. The chromatographic study (TLC) showed that the root extract contains two types of alkaloid compounds represented by harmine and harmaline using a solvent system consisting of (methanol / chloroform / ammonia) (79-20-1)(V). The analysis of antioxidant activity using DPPH and FRAP methods showed that the harmine had a significant activity in capturing free radicals (IC50= 46.89 ± 0.31 µg/ml) with DPPH test, while the crude alkaloid extract had a great ability to reduce Fe 3+ to Fe 2 + , (EC50 = 0.12 ± 0.05 mg/ml) with FRAP test . The results of the anti-inflammatory activity showed For the alkaloids compounds, there were significant differences compared to the control compound represented by diclofenac, the best value was recorded with the harmine compound (EC50= 22.71 ± 0.52 µg/ml). Regarding the anticoagulant activity, the results related to the time effect showed a very important difference with respect to both APTT and PT, where the best values were recorded with harmine, with both tests with (37.94 ± 3.07 seconds), (23.57 ± 2.11 seconds), respectively. The antibacterial activity of different alkaloids extracts was estimated against three reference strains: P. aeruginosa, S. aureus and E. coli using the agar method in nutrient medium. These results showed that the crude alkaline extract of the roots was the strongest against strain S aureus with damping area diameter of 20 mm.